Sequencing and comparative analyses of Aegilops tauschii chromosome arm 3DS reveal rapid evolution of Triticeae genomes.

نویسندگان

  • Jingzhong Xie
  • Naxin Huo
  • Shenghui Zhou
  • Yi Wang
  • Guanghao Guo
  • Karin R Deal
  • Shuhong Ouyang
  • Yong Liang
  • Zhenzhong Wang
  • Lichan Xiao
  • Tingting Zhu
  • Tiezhu Hu
  • Vijay Tiwari
  • Jianwei Zhang
  • Hongxia Li
  • Zhongfu Ni
  • Yingyin Yao
  • Huiru Peng
  • Shengli Zhang
  • Olin D Anderson
  • Patrick E McGuire
  • Jan Dvorak
  • Ming-Cheng Luo
  • Zhiyong Liu
  • Yong Q Gu
  • Qixin Sun
چکیده

Bread wheat (Triticum aestivum, AABBDD) is an allohexaploid species derived from two rounds of interspecific hybridizations. A high-quality genome sequence assembly of diploid Aegilops tauschii, the donor of the wheat D genome, will provide a useful platform to study polyploid wheat evolution. A combined approach of BAC pooling and next-generation sequencing technology was employed to sequence the minimum tiling path (MTP) of 3176 BAC clones from the short arm of Ae. tauschii chromosome 3 (At3DS). The final assembly of 135 super-scaffolds with an N50 of 4.2 Mb was used to build a 247-Mb pseudomolecule with a total of 2222 predicted protein-coding genes. Compared with the orthologous regions of rice, Brachypodium, and sorghum, At3DS contains 38.67% more genes. In comparison to At3DS, the short arm sequence of wheat chromosome 3B (Ta3BS) is 95-Mb large in size, which is primarily due to the expansion of the non-centromeric region, suggesting that transposable element (TE) bursts in Ta3B likely occurred there. Also, the size increase is accompanied by a proportional increase in gene number in Ta3BS. We found that in the sequence of short arm of wheat chromosome 3D (Ta3DS), there was only less than 0.27% gene loss compared to At3DS. Our study reveals divergent evolution of grass genomes and provides new insights into sequence changes in the polyploid wheat genome.

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عنوان ژورنال:
  • Journal of genetics and genomics = Yi chuan xue bao

دوره 44 1  شماره 

صفحات  -

تاریخ انتشار 2017